[Diazinon alters sex hormones, Interferon-gamma, Interleukin-4 and 10 in male Wistar rats]

Agricultural toxins including organochlorine and organophosphorus families cause damages in the various tissues in humans. Diazinon is a non-systemic organophosphate insecticide. This study was carried out to determine the effect of Diazinon on sex hormone, interferon gamma, interleukin-4 and 10 in male rats. In this experimental study 24 adult male Wistar rats were randomly allocated into four groups. Three experimental groups were received Diazinon 5 days per week for one month at 0.3, 3 and 30 mg/kg/bw intraperitoneally, while controls received nothing. Seven days after the last injection, blood samples were obtained and the serum testosterone, FSH, LH, interferon gamma, interleukin-4 and interleukin-10 were measured. Serum level of Interleukin-10 significantly increased in experimental group [30 mg/kg/bw of Diazinon] compared to controls [P<0.05]. Serum level of Interleukin-10 significantly decreased in 0.3 mg/kg/bw and 3mg/kg/bw of Diazinon groups compared to controls [P<0.05]. Interleukin-4 level was only significant in the group receiving 30 mg/kg/bw of Diazinon [P<0.05]. Reduction in interferongamma level was not significant between control and experimental groups. FSH significantly reduced in the three experimental groups in comparison with controls [P<0.05]. Testosterone level was significantly increased in experimental groups compared to control [P<0.05]. Diazinon increases interleukin-10 and testosterone and reduces FSH hormone in the rat

[Preparation and purification of anti immunoglobulin Y antibodies from rabbit]

Background and Objective: Chicken egg yolk antibodies [IgY] play an important role as an alternative to mammalian polyclonal antibodies. They are recently used in medical research for prophylaxis, diagnosis and treatment of diseases. Anti-IgY antibodies are used for immunoassay and therapy. The aim of this study was preparation and purification of anti-immunoglobulin Y antibodies from rabbit serum and confirm of its activity

Methods: IgY and other water-soluble proteins were extracted with acid and chloroform and then thiophilic chromatography [T-gel] was performed and applied for purification of IgY. Two female rabbits were immunized with IgY in Freud's adjuvant and then booster doses were administered, blood samples were collected by times. Ammonium sulfate precipitation, then gel filtration or T-gel chromatography used for purification of Anti-IgY antibodies. Cellulous acetate electrophoresis, double immunodiffusion and ELISA procedures used for confirm of identification and activity

Findings: The purified protein [IgY] was observed in the gamma-globulin region of electrophoresis. The purified antibodies from rabbit serum were observed in the gamma-globulin region, which reacted against IgY in double immunodiffusion and the titer of purified antibody-using ELISA, 1.10000 obtained

Conclusion: These antibodies serve as the basis of many immunoassays for detection and assay of several molecules as anti-immunoglobulin Y reagents